Biosynthesis of phoslactomycins: cyclohexanecarboxylic acid as the starter unit
نویسندگان
چکیده
Phoslactomycins (PLMs) A–F, produced by actinomycetes are polyketide-type antibiotics derived from a hydroxycyclohexanecarboxylic acid or a cyclohexanecarboxylic acid starter unit. Feeding experiments with [2-C]shikimic acid indicated that the C-18 carbon of PLMs comes from C-5 of shikimate. Further feeding studies of cis and trans-3-hydroxy[7-C]cyclohexanecarboxylic acid, [7-C]and [H11]cyclohexanecarboxylic acid have suggested that the starter unit in the PLM biosynthesis is not cis-3hydroxycyclohexanecarboxylate but cyclohexanecarboxylate and that PLM-B is produced initially, and subsequently converted to other analogs by hydroxylation and acylation. q 2003 Elsevier Ltd. All rights reserved.
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Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster.
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